This is really annoying because the data is so nested. Unfortunately it also includes all the directories above the final listed directory. This recrursively downloads all the files. cd /mnt/gluster/data/internal_supp/singleCellSeq/ I am storing the data in internal_supp so that it is easier for PoYuan and me to collaborate. Instead, you need to preface /NGS with /Genomics_Data. There does not appear to be a “Gilad lab folder”. In order to download the data from the command-line, you have to modify the path that Pieter sends. Nick has recently downloaded data from the FGF FTP site.

IMPORTANT: While the Core has looked at your data and found no technical irregularities we urge you to perform data analysis/QC in a timely manner and contact us with any questions/comments you may have within 3 months after the data were made available to you!! Let me know if other files and / or explanations are needed. For access a username and password have been provided to the PI previously (not your CNET ID!!). I have uploaded the data in fastq format to the Genomics Core ftp server data server (gilad Lab folder, folder = /NGS/150320_700819F_0303_AC6WYKACXX-YG-SR100-FC-1). Declining sequencing quality at higher bp-length is (in part) caused by presence of adaptor only sequences (up to 40% at bp 100 according to FastQC). We finished one Flowcell of your Illumina sequencing experiment (4 flowcells SR100).

The first of four flow cells was sequenced at the Functional Genomics Facility (FGF).

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